A FASTQ file is a text file that contains the sequence data from the clusters that pass filter on a flow cell (for more information on clusters passing filter, see the “additional information” section of this bulletin).

The BSSH web uploader allows for files to be uploaded with a maximum size of 250 GB and a maximum number of 16 files per upload. In order to upload multiple samples at once or larger files, BaseSpace …

The following are options to concatenate the FASTQ files from different lanes of the same sequencing run on the NextSeq 500/550, HiSeq series, and NovaSeq 6000 instruments.

MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow. NextSeq 1000/2000 …

The following are options to concatenate the FASTQ files from different lanes of the same sequencing run on the NextSeq 500/550, HiSeq series, and NovaSeq 6000 instruments.

Navigate to the project to which the fastq files will be uploaded. Near the top left, under the project name, select the File icon (which looks like a piece of paper with the corner folded), then select …

You can generate FASTQ files from sequencing data from benchtop instruments even if the run does not complete. To generate FASTQs from an incomplete run in Local Run Manager, follow the steps …

To configure MiSeq Reporter to generate FASTQ files for index reads, follow the steps below: Note: This procedure requires restarting the MiSeq Reporter Service, which requires administrative privileges …

The following directories outline the file paths for the Run Output directory that is copied from the instrument to the Default Output folder for the configured External Storage.

Illumina FASTQ file generation pipelines include an adapter trimming option for the removal of adapter sequences from the 3’ ends of reads. Adapter sequences should be removed from reads because …